Publications
2024
- H. Put1, H. Gerstmans1, H. Vande Capelle1, M. Fauvart*, J. Michiels* and J. Masschelein*. (2024) Bacillus subtilis as a host for natural product discovery and engineering of biosynthetic gene clusters. Natural Product Reports, DOI: 10.1039/D3NP00065F
- J. Čivić, N.R. McFarlane, J. Masschelein and J.N. Harvey. (2024) Exploring the selectivity of cytochrome P450 for enhanced novel anticancer agent synthesis. Faraday Discussions, DOI: 10.1039/D4FD00004H
- C. Cautereels, J. Smets, P. Bircham, D. De Ruysscher, A. Zimmerman, P. De Rijk, J. Steensels, A. Gorkovskiy, J. Masschelein and K. Verstrepen. (2024) Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast. Nature Communications, 15, 1112.
2023
- M.W. Mullowney, K. Duncan, S. Elsayed, N. Garg, N. Martin, D. Meijer, B. Terlouw, F. Biermann, K. Blin, J. Durairaj, M. Gorostiola González, E.J.N Helfrich, F. Huber, S. Leopold-Messer, K. Rajan, T. de Rond, J.A. van Santen, M. Sorokina, M. Balunas, J. van der Hooft, M.A. Beniddir, D. van Bergeijk, L.M. Carroll, C.M. Clark, D.-A. Clevert, C.A. Dejong, C. Du, S. Ferrinho, F. Grisoni, A. Hofstetter, W. Jespers, P. Schwaller, O.V. Kalinina, S.A. Kautsar, H. W. Kim, T.F. Leao, J. Masschelein, E.R. Rees, D. Reker, R. Reher, M. Segler, M. Skinnider, A.S. Walker, B. E. Willighagen, Zdrazil, N. Ziemert, R. Goss, P. Guyomard, A. Volkamer, W.H. Gerwick, H.U. Kim, R. Muller, G. van Wezel, G. Van Westen, A. Hirsch, R. Linington, S.L. Robinson and M. Medema. (2023) Artificial Intelligence For Natural Product Drug Discovery. Nature Reviews Drug Discovery, 22, 895–916.
- E. Vriens1, D. De Ruysscher1, A. Weir, S. Dekimpe, G. Steurs, A. Shemy, L. Persoons, A.R. Santos, C. Williams, D. Daelemans, M.P. Crump, A. Voet, W. De Borggraeve, E. Lescrinier and J. Masschelein. (2023) Polyketide synthase-mediated O-methyloxime formation in the biosynthesis of the oximidine anticancer agents. Angewandte Chemie Int. Ed., 62, e202304476.
- Highlighted as a "Very important paper" by the journal.
- Highlighted in "Hot off the press" Nat. Prod. Rep., 2023, 40, 1590.
- Featured in 'The Brussels Times', 'Het Nieuwsblad', 'Le Soir', 'La Libre', 'Knack' and 'ROB-tv'
2021
- S. Dekimpe and J. Masschelein. (2021) Beyond peptide bond formation: the versatile role of condensation domains in natural product biosynthesis. Natural Product Reports, 38, 1910-1937.
2020
- C.D. Fage1, T. Lathouwers1, M. Vanmeert, L.-J. Gao, K. Vrancken, E.-M. Lammens, A. Weir, R. Degroote, H. Cuppens, S. Kosol, T.J. Simpson, M.P. Crump, C.L. Willis, P. Herdewijn, E. Lescrinier, R. Lavigne, J. Anné and J. Masschelein. (2020) The kalimantacin polyketide antibiotics inhibit fatty acid biosynthesis in Staphylococcus aureus by targeting the enoyl-acyl carrier protein binding site of FabI. Angewandte Chemie Int. Ed, 59, 10549-10556.
- I.T. Nakou, M. Jenner, Y. Dashti, I. Romero-Canelon, J. Masschelein, E. Mahenthiralingam, G.L. Challis. (2020) Genomics-driven discovery of a novel glutarimide antibiotic from Burkholderia gladioli reveals an unusual polyketide synthase chain release mechanism. Angewandte Chemie Int. Ed, 59, 5923145-23153.
- J.A. Davies, F.M. Bull, P.D. Walker, A.N.M. Weir, R. Lavigne, J. Masschelein, T.J. Simpson, P.R. Race, M.P. Crump, C.L. Willis. (2020) Total Synthesis of Kalimantacin A. Organic Letters, 22, 6349-6353.
2019
- J. Masschelein1, P.K. Sydor1, C. Hobson, R. Howe, C. Jones, D.M. Roberts, Z.L. Yap, E. Mahenthiralingam and G.L. Challis. (2019) A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis. Nature Chemistry, 11, 906-912.
- Highlighted in “Hot off the press” Natural Product Reports, 2019, 36, 1614 and Synfacts, 2020, 16, 0225.
- S. Kosol1, A. Gallo1, D. Griffiths1, T.R. Valentic, J. Masschelein, M. Jenner, E.D.L. Santos, L. Manzi, D. Rea, V. Fülop, N.J. Oldham, S.-C. Tsai, G.L. Challis and J.R. Lewandowski. (2019) Structural basis for chain release from the enacyloxin polyketide synthase. Nature Chemistry, 11, 913-923.
- S. Zhou, L. Song, J. Masschelein, F.A.M. Sumang, I.A. Papa, T.O. Zulaybar, A.B. Custodio, D. Zabala, E.P. Alcantara, E.L.C. de los Santos and G.L. Challis. (2019) Pentamycin biosynthesis in Philippine Streptomyces sp. S816: Cytochrome P450-catalysed installation of the C-14 hydroxyl group. ACS Chemical Biology, 14, 1305-1309.
- Highlighted in “Hot off the press” Natural Product Reports, 2019, 36, 1039.
- M. Jenner, X. Jian, Y. Dashti, J. Masschelein, C. Hobson, D.M. Roberts, C. Jones, S. Harris, J. Parkhill, H. A. Raja, N.H. Oberlies, C.J. Pearce, E. Mahenthiralingam and G.L. Challis. (2019) An unusual Burkholderia gladioli double chain-initiating nonribosomal peptide synthetase assembles ‘fungal’ icosalide antibiotics. Chemical Science, 10, 5489-5494.
2018
- G.L. Challis, X. Jian, C. Hobson , J. Masschelein. (2018) Novel Enacyloxin Derivatives. PCT Int. Appl., WO/2018/193273.
2017
- G.L. Challis, D.J. Griffiths, J. Masschelein. (2017) Antimicrobial Agents. PCT Int. Appl., WO/2017/182828.
- I.R.G. Thistlethwaite, F.M. Bull, C. Cui, P.D. Walker, S.-S. Gao, L. Wang, Z. Song, J. Masschelein, R. Lavigne, M.P. Crump, P.R. Race, T.J. Simpson and C.L. Willis. (2017) Elucidation of the relative and absolute stereochemistry of the kalimantacin/batumin antibiotics. Chemical Science, 8, 6196-6201.
- L. Song1, M. Jenner1, J. Masschelein1, C. Jones, M.J. Bull, S.R. Harris, R.C. Hartkoorn, A. Vocat, I. Romero-Canelon, P. Coupland, G. Webster, M. Dunn, R. Weiser, C. Paisey, S.T. Cole, J. Parkhill, E. Mahenthiralingam and G.L. Challis. (2017) Discovery and biosynthesis of gladiolin: a Burkholderia gladioli antibiotic with promising activity against Mycobacterium tuberculosis. Journal of the American Chemical Society, 139, 7974-7981.
- Featured in the Science and Technology section of The Economist (24 June 2017).
- Highlighted in American Chemical Society (14 June 2017).
- Featured in the Science and Technology section of The Economist (24 June 2017).
- J. Masschelein1, M. Jenner1 and G.L. Challis. (2017) Antibiotics from Gram-negative bacteria: a comprehensive overview and selected biosynthetic highlights. Natural Product Reports, 34, 712-783.
- Featured on the front cover.
- Featured on the front cover.
- U.R. Awodi1, J.L. Ronan1, J. Masschelein1, E.L.C. de los Santos and G.L. Challis. (2017) Thioester reduction and aldehyde transamination are universal steps in actinobacterial polyketide alkaloid biosynthesis. Chemical Science, 8, 411-415.
2016
- B. Uytterhoeven, K. Appermans, L. Song, J. Masschelein, T. Lathouwers, C.W. Michiels and R. Lavigne. (2016) Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach. MicrobiologyOpen, 5, 279-286.
2015
- M. Medema, R. Kottmann, P. Yilmaz, M. Cummings, J. Biggins, K. Blin, I. de Bruijn, Y. Chooi, J. Claesen, R. Coates, P. Cruz-Morales, S. Duddela, S. Düsterhus, D. Edwards, D. Fewer, N. Garg, C. Geiger, J. Gomez-Escribano, A. Greule, M. Hadjithomas, A. Haines, E. Helfrich, M. Hillwig, K. Ishida, A. Jones, C. Jones, K. Jungmann, C. Kegler, H. Kim, P. Kötter, D. Krug, J. Masschelein, A. Melnik, S. Mantovani, E. Monroe, M. Moore, N. Moss, H. Nützmann, G. Pan, A. Pati, D. Petras, F. Reen, F. Rosconi, Z. Rui, Z. Tian, N. Tobias, Y. Tsunematsu, P. Wiemann, E. Wyckoff, X. Yan, G. Yim, F. Yu, Y. Xie, B. Aigle, A. Apel, C. Balibar, E. Balskus, F. Barona-Gómez, A. Bechthold, H. Bode, R. Borriss, S. Brady, A. Brakhage, P. Caffrey, Y. Cheng, J. Clardy, R. Cox, R. DeMot, S. Donadio, M. Donia, W. van der Donk, P. Dorrestein, S. Doyle, A. Driessen, M. Ehling-Schulz, K. Entian, M. Fischbach, L. Gerwick, W. Gerwick, H. Gross, B. Gust, C. Hertweck, M. Höfte, S. Jensen, J. Ju, L. Katz, L. Kaysser, J. Klassen, N. Keller, J. Kormanec, O. Kuipers, T. Kuzuyama, N. Kyrpides, H. Kwon, S. Lautru, R. Lavigne, C. Lee, B. Linquan, X. Liu, W. Liu, A. Luzhetskyy, T. Mahmud, Y. Mast, C. Méndez, M. Metsä-Ketelä, J. Micklefield, D. Mitchell, B. Moore, L. Moreira, R. Müller, B. Neilan, M. Nett, J. Nielsen, F. O'Gara, H. Oikawa, A. Osbourn, M. Osburne, B. Ostash, S. Payne, J. Pernodet, M. Petricek, J. Piel, O. Ploux, J. Raaijmakers, J. Salas, E. Schmitt, B. Scott, R. Seipke, B. Shen, D. Sherman, K. Sivonen, M. Smanski, M. Sosio, E. Stegmann, R. Süssmuth, K. Tahlan, C. Thomas, Y. Tang, A. Truman, M. Viaud, J. Walton, C. Walsh, T. Weber, G. van Wezel, B. Wilkinson, J. Willey, W. Wohlleben, G. Wright, N. Ziemert, C. Zhang, S. Zotchev, R. Breitling, E. Takano, F. Glöckner. (2015) Minimum information about a biosynthetic gene cluster. Nature Chemical Biology, 11, 625-631.
- J. Masschelein, C. Clauwers, K. Stalmans, Y. Briers, A. Aertsen, C. Michiels and R. Lavigne. (2015) The zeamine antibiotics affect the integrity of bacterial membranes. Applied and Environmental Microbiology, 81, 1139-1146.
- J. Masschelein, C. Clauwers, U.R. Awodi, K. Stalmans, W. Vermaelen, E. Lescrinier, A. Aertsen, C. Michiels, G.L. Challis and R. Lavigne. (2015) A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibiotics. Chemical Science, 6, 923-929.
2014
- R. Van Houdt, D. Van der Lelie, J. Izquierdo, A. Aertsen, J. Masschelein, R. Lavigne, C. Michiels and S. Taghavi. (2014) Genome sequence of Serratia plymuthica RVH1, isolated from a raw vegetable processing line. Genome Announcements, 2, e00021-14.
2013
- A. Haines, Z. Song, R. Farmer, C. Williams, J. Hothersall, E. Ploskon, P. Wattana-Amorn, E. Stephens, E. Yamada, R. Gurney, Y. Takebayashi, J. Masschelein, R. Cox, R. Lavigne, C. Willis, T. Simpson, J. Crosby, P. Winn, C. Thomas, M. Crump. (2013) A conserved motif flags acyl carrier proteins for β-branching in polyketide synthesis. Nature Chemical Biology, 9, 685-692.
- J. Masschelein, W. Mattheus, L.-J. Gao, P. Moons, R. Van Houdt, B. Uytterhoeven, C. Lamberigts, E. Lescrinier, J. Rozenski, P. Herdewijn, A. Aertsen, C. Michiels and R. Lavigne. (2013) A PKS/NRPS/FAS hybrid gene cluster from Serratia plymuthica RVH1 encoding the biosynthesis of three broad spectrum, zeamine-related antibiotics. PloS One, 8, e54143.
2010
- W. Mattheus, J. Masschelein, L.-J. Gao, P. Herdewijn, B. Landuyt, G. Volckaert and R. Lavigne. (2010) The kalimantacin/batumin biosynthesis operon encodes a self-resistance isoform of the FabI bacterial target. Chemistry & Biology, 17, 1067-1071.
- W. Mattheus, L.-J. Gao, P. Herdewijn, B. Landuyt, J. Verhaegen, J. Masschelein, G. Volckaert and R. Lavigne. (2010) Isolation and purification of a new kalimantacin/batumin-related polyketide antibiotic and elucidation of its biosynthesis gene cluster. Chemistry & Biology, 17, 17, 149-159.